Light-triggered beta-hairpin folding and unfolding

PNAS, 2007, vol. 104, no. 40, 15729-34 published on 02.10.2007, online article
A light-switchable peptide is transformed with ultrashort pulses from aß-hairpin to an unfolded hydrophobic cluster and vice versa. The structural changes are monitored by mid-IR probing. Instantaneous normal mode analysis with a Hamiltonian combining density functional theory with molecular mechanics is used to interpret the absorption transients. Illumination of the b-hairpin state triggers an unfolding reaction that visits several intermediates and reaches the unfolded state within a few nanoseconds. In this unfolding reaction to the equilibrium hydrophobic cluster conformation, the system does not meet significant barriers on the free-energy surface. The reverse folding process takes much longer because it occurs on the time scale of 30 micro s The folded state has a defined structure, and its formation requires an extended search for the correct hydrogen-bond pattern of the ß-strand.

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