TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles
Org. Biomol. Chem., 2019, DOI: 10.1039/c9ob00508k
Org. Biomol. Chem., online article
We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.